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mal  (Vector Laboratories)


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    Structured Review

    Vector Laboratories mal
    Mal, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maackia+amurensis+lectin/Fluorescein+labeled+Maackia+Amurensis+Lectin+I+(MAL+I)/pm42037410-272-24-27
    Average 93 stars, based on 37 article reviews
    mal - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic cats.
    Article Snippet: Sections were then blocked using goat serum (Thermo Fisher Scientific, catalog# PCN5000) diluted 1:40 with TBS and further blocked using a Streptavidin/Biotin Blocking Kit (Vector Laboratories, SP2002), following the manufacturer's guidelines. .. The sections were incubated with Sambucus Nigra Lectin (SNA), Fluorescein-labeled (Vector Laboratories, FL13012), Maackia Amurensis Lectin II (MAA II), Unconjugated (Vector Laboratories, L-1260-2), and Maackia Amurensis Lectin I (MAA I), Fluorescein (Vector Laboratories, FL13112). ..

    Article Title:
    Article Snippet: Non-specific binding was blocked by incubating cells with Carbo-free Blocking Solution Kit (Vectorlabs, SP-2001) and washed three times in PBS. .. Cells were incubated at room temperature for 45 min with 10 μg mL-1 of Alexa Fluor 488-labeled Helix pomatia agglutin (HPA, ThermoFisher) for GalNAc staining, Cy3-labeled Sambucus nigra agglutinin (SNA, Vectorlabs) for α2,6-SA staining, and fluoresceinlabeled Maackia amurensis lectin (MAL, Vectorlabs) for α2,3-SA staining, and washed three times with TPBS (PBS + 0.05% Tween 20). ..

    Article Title: N-acetylneuraminic acid modulates SQSTM1/p62 sialyation-mediated ubiquitination degradation contributing to vascular endothelium dysfunction in experimental atherosclerosis mice.
    Article Snippet: .. The total cell lysate was incubated with Maackia Amurensis Lectin I (MALI) (Vectorlabs, #FL-1311-2) or Sambucus Nigra Lectin (SNA) (Vectorlabs, #FL-1301-2) in a rotator at 4 C overnight. .. Then add BeyoMagTM Streptavidin Magnetic Beads (Beyotime, #P2151) and continue incubating at 4 C for 4–16 h. Sialylated glycoproteins were enriched by centrifugation and washed 3 times with cold lectin buffer.

    Glycoproteomics:

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines.
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines.
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    Binding Assay:

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines.
    Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Maackia Amurensis Lectin I (MAL-I) (Vector Laboratories), and Sambucus Nigra Lectin (SNA, EBL) (Vector Laboratories), while binding to Siglecs was similarly assessed by ELISA with Siglec-Fc chimeras. ..

    other:

    Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats.
    Article Snippet: Biotinylated lectins, including Aleuria aurantia lectin (AAL, 1:5000, Vector Laboratories), Lens culinaris agglutinin (LCA, 1:5000, Vector Laboratories), Sambucus nigra agglutinin (SNA, 1:10000, Vector Laboratories), Maackia amurensis lectin (MAL, 1:3000, Vector Laboratories), Phaseolus vulgaris Erythroagglutinin (PHA-E, 1:3000, Vector Laboratories), Phaseolus vulgaris Leucoagglutinin (PHA-L, 1:3000, Vector Laboratories) and Concanavalin A (ConA, 1:10000, Vector Laboratories) were purchased from Vector Laboratories Inc. (Burlingame, CA, USA) AR TIC LE IN PR ES S (Table 1).

    Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats
    Article Snippet: Biotinylated lectins, including Aleuria aurantia lectin (AAL, 1:5000, Vector Laboratories), Lens culinaris agglutinin (LCA, 1:5000, Vector Laboratories), Sambucus nigra agglutinin (SNA, 1:10000, Vector Laboratories), Maackia amurensis lectin (MAL, 1:3000, Vector Laboratories), Phaseolus vulgaris Erythroagglutinin (PHA-E, 1:3000, Vector Laboratories), Phaseolus vulgaris Leucoagglutinin (PHA-L, 1:3000, Vector Laboratories) and Concanavalin A (ConA, 1:10000, Vector Laboratories) were purchased from Vector Laboratories Inc. (Burlingame, CA, USA) (Table ).

    Whole Genome Amplification:

    Article Title: Enzymatic tailoring of anionic glycans for characterising lectin and antibody specificities in a microarray format.
    Article Snippet: Donor substrates 3'- phosphoadenosine-5'-phosphosulphate (PAPS), cytidine 5′-monophosphate-N-acetyl-- neuraminic acid (CMP-Neu5Ac), cytidine 5′-monophosphate-N-glycolyl--neuraminic acid disodium salt (CMP-Neu5Gc), guanosine 5'-diphospho--L-fucose disodium salt (GDP-Fuc), uridine-5-diphospho-N-acetyl--galactosamine sodium salt (UDP-GalNAc), uridine-5- Jo urn al Pr e-p roo f 10 diphospho--glucuronic acid trisodium salt (UDP-GlcA), uridine-5-diphospho--galactose (UDP-Gal) were purchased from R&D Systems (PAPS), Jennewein Biotechnologie GmbH, Rheinbreitbach, Germany (CMP-Neu5Ac and GDP-Fuc); ChemilyBio (CMP-Neu5Gc) and SigmaAldrich (all others) respectively. .. Lectins and Primary Antibodies Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg /ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra/Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein labelled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories, Newark, CA, US. ..

    Staining:

    Article Title:
    Article Snippet: Non-specific binding was blocked by incubating cells with Carbo-free Blocking Solution Kit (Vectorlabs, SP-2001) and washed three times in PBS. .. Cells were incubated at room temperature for 45 min with 10 μg mL-1 of Alexa Fluor 488-labeled Helix pomatia agglutin (HPA, ThermoFisher) for GalNAc staining, Cy3-labeled Sambucus nigra agglutinin (SNA, Vectorlabs) for α2,6-SA staining, and fluoresceinlabeled Maackia amurensis lectin (MAL, Vectorlabs) for α2,3-SA staining, and washed three times with TPBS (PBS + 0.05% Tween 20). ..



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    Vector Laboratories biotin mal ii
    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections <t>using</t> <t>MAL</t> <t>II</t> lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .
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    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections <t>using</t> <t>MAL</t> <t>II</t> lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .
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    Image Search Results


    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Journal: Frontiers in Pharmacology

    Article Title: Mycobacterium tuberculosis infection drives osteoclast overactivation via α2,3-Sialylation to promote pathological bone destruction

    doi: 10.3389/fphar.2026.1738896

    Figure Lengend Snippet: α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Article Snippet: Biotin MAL-II , Vector laboratories , Cat# B-1265-1.

    Techniques: Activity Assay, Comparison, Infection, Quantitative Proteomics, Immunofluorescence, Staining, Fluorescence, In Vitro, Cell Culture, Expressing, Two Tailed Test