mal (Vector Laboratories)
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Mal, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maackia+amurensis+lectin/Fluorescein+labeled+Maackia+Amurensis+Lectin+I+(MAL+I)/pm42037410-272-24-27
Average 93 stars, based on 37 article reviews
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Incubation:Article Title: Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic cats. Article Snippet: Sections were then blocked using goat serum (Thermo Fisher Scientific, catalog# PCN5000) diluted 1:40 with TBS and further blocked using a Streptavidin/Biotin Blocking Kit (Vector Laboratories, SP2002), following the manufacturer's guidelines. .. The sections were incubated with Sambucus Nigra Lectin (SNA), Fluorescein-labeled (Vector Laboratories, FL13012), Maackia Amurensis Lectin II (MAA II), Unconjugated (Vector Laboratories, L-1260-2), and Article Title: Article Snippet: Non-specific binding was blocked by incubating cells with Carbo-free Blocking Solution Kit (Vectorlabs, SP-2001) and washed three times in PBS. .. Cells were incubated at room temperature for 45 min with 10 μg mL-1 of Alexa Fluor 488-labeled Helix pomatia agglutin (HPA, ThermoFisher) for GalNAc staining, Cy3-labeled Sambucus nigra agglutinin (SNA, Vectorlabs) for α2,6-SA staining, and fluoresceinlabeled Article Title: N-acetylneuraminic acid modulates SQSTM1/p62 sialyation-mediated ubiquitination degradation contributing to vascular endothelium dysfunction in experimental atherosclerosis mice. Article Snippet: .. The total cell lysate was incubated with Glycoproteomics:Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines. Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Enzyme-linked Immunosorbent Assay:Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines. Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Binding Assay:Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), Article Title: Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines. Article Snippet: .. The presence and functionality of the sialylated glycan was confirmed by ELISA with α2,3-specific Lectenz (Lectenz Bio), other:Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats. Article Snippet: Biotinylated lectins, including Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats Article Snippet: Biotinylated lectins, including Whole Genome Amplification:Article Title: Enzymatic tailoring of anionic glycans for characterising lectin and antibody specificities in a microarray format. Article Snippet: Donor substrates 3'- phosphoadenosine-5'-phosphosulphate (PAPS), cytidine 5′-monophosphate-N-acetyl-- neuraminic acid (CMP-Neu5Ac), cytidine 5′-monophosphate-N-glycolyl--neuraminic acid disodium salt (CMP-Neu5Gc), guanosine 5'-diphospho--L-fucose disodium salt (GDP-Fuc), uridine-5-diphospho-N-acetyl--galactosamine sodium salt (UDP-GalNAc), uridine-5- Jo urn al Pr e-p roo f 10 diphospho--glucuronic acid trisodium salt (UDP-GlcA), uridine-5-diphospho--galactose (UDP-Gal) were purchased from R&D Systems (PAPS), Jennewein Biotechnologie GmbH, Rheinbreitbach, Germany (CMP-Neu5Ac and GDP-Fuc); ChemilyBio (CMP-Neu5Gc) and SigmaAldrich (all others) respectively. .. Lectins and Primary Antibodies Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg /ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Staining:Article Title: Article Snippet: Non-specific binding was blocked by incubating cells with Carbo-free Blocking Solution Kit (Vectorlabs, SP-2001) and washed three times in PBS. .. Cells were incubated at room temperature for 45 min with 10 μg mL-1 of Alexa Fluor 488-labeled Helix pomatia agglutin (HPA, ThermoFisher) for GalNAc staining, Cy3-labeled Sambucus nigra agglutinin (SNA, Vectorlabs) for α2,6-SA staining, and fluoresceinlabeled |
